Cellular Evidence of Hepato- and Neuro-Protective Effect of Fish Press Juices against Hydrogen Peroxide-induced Oxidative Stress

Mostafa Waly¹, Mohamed Al-Bulishi¹ , Jamal N Al-Sabahi¹, Bassam Soussi^2,3*

¹Department of Food Science and Nutrition, ²UNESCO Chair, Marine Biotechnology, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, Sultanate of Oman. ³Institute for Clinical Sciences, Sahlgren’s University Hospital, Goteborg University, Sweden

*Corresponding Author:Prof. Bassam Soussi, [email protected]

ABSTRACT
Background: Oxidative stress induced by free radicals and reactive oxygen species, depletes intracellular glutathione (GSH) and inhibits antioxidant enzymes. Consumption of antioxidant-rich foods reduces cellular oxidative stress and protects against health related problems. Objective: This study aimed to (1) measure the antioxidants contents of the press juices (PJs) extracted from Hamour and King Fish and (2) Evaluate the PJs effect on oxidative stress induced by hydrogen peroxide, using human cultured neuronal cells (SH-SY5Y) and hepatic cells (HepG2). Results: PJs analysis revealed the presence of significant amounts of antioxidants, vitamin C and GSH in both fish species. The total antioxidant capacity (TAC) of Hamour PJ was significantly higher than that of Kingfish PJ. Hydrogen peroxide (H₂O₂)-induced oxidative stress in human cultured neuronal cells (SH-SY5Y) and hepatic cells (HepG2) as presented by GSH depletion and antioxidant enzymes inhibition. PJs protect againstH₂O₂-induced oxidative cellular insults. Conclusion: PJs from Hamour and Kingfish, locally available fishes in Oman, are powerful dietary sources for antioxidants and protect against oxidative stress. Further experimental animals studies are required to further investigate the in vivo effects of PJs against oxidative stress and health related problems.

Key words: Antioxidants, Oxidative Stress, Fish Press Juices, Human Cell Lines

Waly MI, Al-Bulishi M, Al-Sabahi JN, Soussi B. Cellular Evidence of Hepato- and Neuro-Protective Effect of Fish Press Juices against Hydrogen Peroxide-induced Oxidative Stress. Canad J Clin Nutr 2015; 3 (1): 32-43

DOI: http://dx.doi.org/10.14206/canad.j.clin.nutr.2015.01.04