Involvement of Chitosan-Cu Complex as Chemoprevention of Experimentally Carcinogenesis in Mice

Gamal Mohamed Fathy Edrees¹, Maher Amer Ali Amer^1*, HanaaAli Hassan¹, Mostafa Al-Tonbary^1
1Zoology Department, Faculty of Science, Mansoura University, Egypt
*Corresponding Author:Professor Maher Amer. Email: [email protected]

ABSTRACT
Background: Chemoprevention is an extremely promising strategy for cancer prevention.Objective: The role of chitosan-cu complex (200 mg/kg) on oxidative stress induced by Erlich ascites carcinoma [EAC] (0.2X106 cells) in female mice was evaluated. Subjects and Methods: Eighty eight female Swiss albino mice weighing 23 ±3 g were divided into four equal groups, Control group (saline solution administrated orally at dose 200 mg/kg 16 days), Chitosan-Cu complex group (Chitosan-Cu complex administered orally at a dose of 200 mg/kg 16 days). Tumour induced group (Ehrlich ascites carcinoma cells were injected intra dermal the fourth day at dose 0.2×106 cells, Chitosan-Cu complex and tumour (Chitosan-Cu complex administered orally at adose of 200 mg/kg from the first day to the last day + Erlich ascites carcinoma. Results:Tumour size, argimase activity, α L-fucosidase and BL2 were significantly exceeded in EAC treated mice. In addition P53 level and Go/1 significantly decreased. These abnormalities are accompanied by increased the lipid peroxidation product (MDA) and decline in total antioxidant enzyme activity as well as GSH level,and mineral alteration. Conclusion: It can be concluded that the mice pretreated with chitosan-Cu complex then with EAC reveal marked protection as antitumor substance.

Key words: Chitosan Copper Complex, Ehrlich Ascites Carcinoma, Antioxidant

EdreesGMF, Amer MAA, Hassan HA, Al-Tonbary M. Involvement of Chitosan-Cu complex as chemoprevention of experimentally carcinogenesis in mice.Canad J ClinNutr 2014; 2 (2): 4-21

DOI: http://dx.doi.org/10.14206/canad.j.clin.nutr.2014.02.02