Genetic Variation of Interleukin-6 -174 G>C Polymorphism with Cervical Cancer Susceptibility in North Indian Population: A Case Control Study

Pushpendra D. Pratap1, Syed Tasleem Raza1*, Ghazala Zaidi2, Shipra Kunwar3, Mark Rector Charles1, Ale Eba1, Muneshwar Rajput1

1Central Research Laboratory, Molecular Diagnostic Unit, Department of Biochemistry, ERA’S Lucknow Medical College, ERA University, Lucknow-226003, Uttar Pradesh, India. 2Department of Allied Health Sciences ERA University, Lucknow-226003, Uttar Pradesh, India. 3Department of Obstetrics and Gynecology ERA’S Lucknow Medical College, ERA University, Lucknow-226003, Uttar Pradesh, India.

*Corresponding author: Professor Syed Tasleem Raza, Department of Biochemistry, ERA’S Lucknow Medical College, ERA University, Uttar Pradesh, India. E-mail: tasleem24@gmail.com

ABSTRACT
Background: Cervical carcinoma is the second utmost common malignant disease in women around the world. HPV was officially designated as the causative agent of cervical cancer. Apart from HPV 16, 18 inflammation plays a major role in the pathogenesis of cervical cancer. IL-6 encodes a protein that leads to inflammation and retains immune homeostasis. Therefore, this case-control study was planned to validate whether polymorphism in inflammation related gene interleukin-6 is linked with cervical cancer susceptibility. Subjects & Methods: We recruited 392 subjects, including 192 histologically validated cases with cervical cancer and 200 age, ethnicity matched healthy controls for this study. IL-6 -174G>C polymorphism was genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) approach. The IL-6 genotype’s frequencies were determined using co-dominant, dominant, and recessive genetic models. The odds ratio (OR) and 95% confidence interval were used to determine the strength of the associations (CI). Results: When compared to controls, the IL-6 174G>C gene polymorphism was linked to a substantially increased cervical cancer risk (OR: 1.854, CI: 1.188-2.895; P = 0.006). Similarly, the GC genotype was linked to a higher cervical cancer risk with tumor stage (OR: 1.648, CI: 0.885-3.067; P = 0.115; OR: 1.963, CI 1.202-3.209; P = 0.007). According to the results of a logistic regression study, the IL-6 -174C allele was independently linked to increased cervical cancer risk (OR: 1.551, CI: 1.07-2.25, P = 0.02). Conclusion: The increased occurrence of the C allele and G/C genotype in patients compared to controls indicates that the IL-6 -174 G>C polymorphism may affect cervical cancer susceptibility.

Keywords: Cervical cancer, Gene polymorphism, Genetic susceptibility, Interleukin-6, Inflammation.

Citation: Pushpendra D. Pratap, Syed Tasleem Raza, Ghazala Zaidi, Shipra Kunwar, Mark Rector Charles, Ale Eba, Muneshwar Rajput. Genetic Variation of Interleukin-6 -174 G>C Polymorphism with Cervical Cancer Susceptibility in North Indian Population: A Case Control Study. Canadian Journal of Clinical Nutrition. 2021; 9 (2): 51-63.

DOI: https://dx.doi.org/10.14206/canad.j.clin.nutr.2021.02.05

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