Protective Effect of Glutathione Feeding against Azoxymethane-induced Oxidative Stress in Rat Colon

Luca Antonelli1*, Arianna Paterlini2, Lucia Carrion3

1Verona University-Italy, 2Parma University-Italy, 3Madrid University-Spain

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Background: Azoxymethane (AOM) is a potent carcinogenic agent, which is frequently used in experimental animals to induce colon cancer. Objective: This study was planned to assess the protective effect of glutathione (GSH) against AOM-induced oxidative stress in colonic tissues of male rats which were divided into four groups, the first group was used as a control, the second group was received  two intraperitoneal injections of AOM, the third group was fed GSH at a dose of 10 mg/kg body weight/day, the fourth group was injected AOM simultaneously with GSH feeding. After six weeks, all rats were sacrificed and colon tissues were dissected and homogenized for biochemical assessment of oxidative stress. Results: AOM injection showed marked elevation content of lipid peroxidation and marked reduction of antioxidant enzymes activities (catalase,  glutathione peroxidase, glutathione reductase, glutathione-S transferase and superoxide dismutase) in the homogenate of colonic tissues. Treatment with GSH alone did not cause any significant changes of these parameters as compared to control group. When using GSH, the AOM-mediated oxidative stress was greatly abolished with a significant reduction in lipid peroxidation level and elevation in the catalase, glutathione  peroxidase and glutathione-S-transferase  activities. Conclusion: It is suggested that GSH may be used as a dietary antioxidant to improve the oxidative stress of colonic toxicity induced by AOM.

Keywords: Glutathione, Azoxymethane, Colon Cancer, Experimental Animals

Citation: Antonelli L, Paterlini A, Carrion L. Protective effect of glutathione feeding against azoxymethane-induced oxidative stress in rat colon. Canad J Clin Nutr 2016; 4 (2): 65-71.