Flow Cytometric, Histological and Ultrastructural Evaluation of the Anti-toxic Effect of Ethanolic Extract of Ficus carica Leaves against CCl4-induced Hepatotoxicity: An In Vivo Study

Amira O. Abd El-Azim*, Eman A. El-Shabasy
Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt
*Corresponding Author E-mail address: amirahosman2000@yahoo.com

ABSTRACT
Objective: Till now, there is no published research addressed the influence of Ficus carica leaves extract on T cell development and/or cell cycle parameters expression in induced hepatotoxicity animals. Therefore, this article spotlighted the influence of Ficus carica leaves’ extract on carbon tetrachloride (CCl4) induced hepatotoxicity, T cell inequity, and cell cycle disturbance. Moreover, these findings were proved histologically, histochemically, and ultrastructurally. Materials & Methods: Four groups of 45-60 days old rats each with 5 animals were divided into; control, extract intragastric injected, CCl4 intraperitoneal injected, and CCl4 plus extract injected groups. At the end of the experiment, all animals were humanely killed; liver tissues were collected and examined histologically, histochemically, and ultrastructurally. In addition, flow cytometry technique was processed to assess CD4+T, CD8+T, Transforming growth factor beta1 (TGFβ1) cells and cell cycle phases. Results: CCl4 induced hepatotoxicity illustrated in necrosis and lymphocytic infiltration were evident in H&E stain, Masson trichrome stain, and at a cellular level using an electron microscope. Besides, flow cytometric analysis confirmed the previous results with CD4+T, CD8+T cells, TGFβ1 and cell cycle parameters, assured  and explained the ameliorating effect of Ficus carica leaves’ extract on hepatic cells. Conclusion: Our findings support that Ficus carica leaves’ extract possesses a valuable efficiency to protect hepatocytes against toxic agents like CCl4 properly referring to apoptotic incidences.

Keywords: CCl4, Ficus carica leaves, Liver, CD4, CD8, TGFβ1

Citation: Abd-Elazim AO, El-Shabasy EA. Flow Cytometric, Histological and Ultrastructural Evaluation of the Anti-toxic Effect of Ethanolic Extract of Ficus carica Leaves against CCl4-induced Hepatotoxicity: An In Vivo Study. Canad J Clin Nutr 2018; 6 (1): 55-80.

DOI: http://dx.doi.org/10.14206/canad.j.clin.nutr.2018.01.05

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