Antioxidant, Antiproliferative, Immunomodulatory, Antimicrobial and Functional Properties of wild Mushroom (Coprinus atramentarius) β-glucan Extract as affected by γ-irradiation Treatment

Asma Ashraf Khan1, Adil Gani1*, F.A. Masoodi1, F. A. Khanday2

1Department of Food Science and Technology, University of Kashmir, Srinagar, India, 190006
2Department of Biotechnology, University of Kashmir, Srinagar, India, 190006
*Correspondence Email Address: adil.gani@gmail.com

ABSTRACT
Background: This study was carried out to evaluate the effect of γ-irradiation on the structural, thermal, antioxidant, antiproliferative, immunomodulatory, antimicrobial and functional properties of β-glucan obtained from the wild variety of mushroom, Coprinus atramentarius. Material and Methods: The structural characterization of the irradiated Coprinus β-glucan was performed using attenuated total reflection – Fourier transform infrared spectroscopy (ATR-FTIR) whereby the β-linkage showed an increased shifting from 874 to 927 cm-1. Results:  The thermal properties studied using DSC revealed that as the irradiation dose increases from 5-50 kGy; there was a corresponding increase in the gelatinization temperature from 25.61 to 58.960C (TO) and 63.76 to 71.380C(TP) Treatment with γ-irradiation enhanced all related antioxidant properties like DPPH, reducing power, metal chelating ability of ferrous ion, ABTS & inhibition of lipid peroxidation (P < 0.05) in a dose dependent manner. The antiproliferative and the immunomodulatory activity of the Coprinus β-glucan also showed a corresponding increase with an increase in the irradiation dose. The antibacterial activity of Coprinus β-glucan against different pathogens was also enhanced with an increase in the irradiation dose from 5 to 50 kGy. Moreover, the functional properties of irradiated mushroom β-glucan were effectively influenced by the action of gamma irradiation wherein the swelling power decreases with an increase in the irradiation dose while fat binding capacity, emulsifying properties, foaming properties and bile acid binding capacity all increased with higher dose.

Conclusion: Therefore, γ-irradiation can be an effective strategy to enhance and improve the functional properties of mushroom β-glucan.
Keywords: Antioxidant, Antiproliferative, Coprinus β-glucan, Immunomodulatory, Irradiation, Structural analysis.
Citation: Asma Ashraf Khan, Adil Gani, F.A. Masoodi, F. A. Khanday. Antioxidant, Antiproliferative, Immunomodulatory, Antimicrobial and Functional Properties of wild Mushroom (Coprinus atramentarius) β-glucan Extract as affected by γ-irradiation Treatment. Canadian Journal of Clinical Nutrition. 2020; 8 (2): 107-134.
DOI: https://dx.doi.org/10.14206/canad.j.clin.nutr.2020.02.08

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